![]() ![]() Low risk to community-effective treatment, preventive available: Bacillus cereus, Salmonella, Shigella, Hepatitis A, B, C, Rubella, ChikungunyaīSL 3 (high individual, low community risk)Įxotic or indigenous agent-potentially transmit disease mainly via aerosols. Effective treatment, preventive and control measures, are readily available. ![]() Infectious risk is via direct contact, ingestion, or inhalation. ![]() coli, Pseudomonas, Baculovirus of insects, AspergillusīSL 2 (moderate individual risk, limited community, livestock or environment risk)Ĭausing human or animal disease but noncontaminant to laboratory researchers, technicians, communities, livestock, or environment. ![]() Implausible to cause significantly human disease or animal disease of veterinary. When conducting works with high transmissible agents, the risk of aerosol transmission is so high, so microbiology laboratories must follow BSL 3 practices.īSL 1 (low individual and community risk) BSL 2 practices are mostly followed by clinical microbiology laboratories. The BSLs are categorized into BSL 1 to BSL 4, and the risk associated with every BSL increases with the infectious microbes encountered. BSLs have standard guidelines that described the proper containment equipment, services, and procedures for apply by laboratory researchers/scientists/technicians/students. The proper design and work structures, safety equipment, and controlling practices should enhance the laboratory personnel and safety practices.Ĭliffe ( 2016) has mentioned that many biosafety levels (BSLs) have been developed for laboratories to enhance the protection levels of environment and staff. A microbiologist/scientist, and knowledgeable laboratory techniques, safety procedures, and hazards associated with handling transferrable agents will accountable for the comportment of research with infectious agents or materials. All laboratories are required to develop a biosafety or operations manual that categorizes the hazards that may be met, and that specifies the proper practices and techniques to reduce/or minimize the hazard exposures. Personnel working with transferable agents are acquainted with hazards that require the proper training and skillful practices for handling such materials. The basics of containments are safety equipment, microbiological practices, and skill safeguards that defend lab workers, environment, and examiners/public from exposure to transferable microbes that are properly handled and kept in the laboratory. Aseptic techniques have the following objectives: (1) to acquire the knowledge of aseptic technique in the field of microbes, (2) to avoid the contamination of cultures from undesirable microbes in the laboratory, (3) to subculture (transfer cultures from one media by inoculating into another media), (4) to isolate pure culture from mixed culture, and (5) to inhibit lab microbes from being distributed in the environment and/or contaminating the investigator (, 2011).īiosafety comprises addressing of the safe handling and containment of transferable microorganisms and unwarranted biomaterials. Appropriate aseptic techniques prevent microbes from unintentionally released into the environment and/or contaminating lab user in the laboratory. Aseptic technique commonly maintains pure stock cultures and single spore culture while transferring cultures into fresh media. Using the proper aseptic technique can significantly reduce/minimize the risk of contamination. As example, airborne microbes (e.g., fungi) handpicked from the surveyor’s health, the lab benchtop, unsterilized glassware and equipment, dust, and other areas, thus interfering to get proper experiment results. Proper aseptic technique has prevented the cultures contamination from inborn and outborn microbes in the environment. Mycologist/microbiologists must follow aseptic techniques for multiplicity procedures such as screening of isolates/strains, pure cultures, slant cultures, single spore cultures, microbes transferring cultures, inoculating media, and conducting several microbiological experiments. It is a compulsory laboratory skill to conduct research related in the field of microbiology. Aseptic technique is a method that involves target-specific practices and procedures under suitably controlled conditions to reduce the contamination from microbes. ![]()
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